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==dsDNA Qubit== Gonna try a double stranded DNA measurement in Qubit to see if there is any signal. <ol> <li>Annealing</li> <ol type="A"> <li>Mix two uL reaction, 2 uL 100 uM Bglii + primer, and 6 uL nfH2O for samples XA-XF (NEB 3hr); include one XA with 2 uL 100 uM Bglii - primer</li> <li>Incubate at 95C for 5 min</li> <li>Incubate at 50C for 10 minutes</li></ol> <li>Qubit</li> <ol type="A"> <li>Mix 1990 uL Qubit dsDNA buffer and 10 uL Qubit dsDNA dye</li> <li>Add 190 uL mix and 10 uL sample for S1 and S2 standards</li> <li>Add 199 uL mix and 1 uL sample for samples; include XA without bglii primer (8 samples total)</li> <li>Incubate in dark 5 minutes</li> <li>Measure in Qubit; use new calibration</li></ol> {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> |- style="background-color:#66FF66;font-size:13pt;font-weight:bold" align="center" | width="140" height="39" | Sample Name | width="280" | Condition | width="85" | ug/mL | width="85" | ng/mL | width="85" | bp | width="85" | nM |- style="font-size:13pt" | height="17" valign="bottom" | XA | align="center" valign="bottom" | NEB RCA 3 hrs CP (LLRC) | align="center" align="center" valign="bottom" | 45.5 | align="center" valign="bottom" | | align="center" align="center" valign="bottom" | 188 | align="center" align="center" valign="bottom" | 733.4 |- style="background-color:#BFBFBF;font-size:13pt" | height="17" valign="bottom" | XB | align="center" valign="bottom" | NEB RCA 3 hrs CP (LLRC*) | align="center" align="center" valign="bottom" | 64 | align="center" valign="bottom" | | align="center" align="center" valign="bottom" | 188 | align="center" align="center" valign="bottom" | 1031.59 |- style="font-size:13pt" | height="17" valign="bottom" | XC | align="center" valign="bottom" | NEB RCA 3 hrs CP (LLRC***) | align="center" align="center" valign="bottom" | 74.7 | align="center" valign="bottom" | | align="center" align="center" valign="bottom" | 188 | align="center" align="center" valign="bottom" | 1204.06 |- style="background-color:#BFBFBF;font-size:13pt" | height="17" valign="bottom" | XD | align="center" valign="bottom" | NEB RCA 3 hrs LP (LLRC) | align="center" align="center" valign="bottom" | 65.1 | align="center" valign="bottom" | | align="center" align="center" valign="bottom" | 188 | align="center" align="center" valign="bottom" | 1049.32 |- style="font-size:13pt" | height="17" valign="bottom" | XE | align="center" valign="bottom" | NEB RCA 3 hrs LP (LLRC*) | align="center" align="center" valign="bottom" | 47.7 | align="center" valign="bottom" | | align="center" align="center" valign="bottom" | 188 | align="center" align="center" valign="bottom" | 768.86 |- style="background-color:#BFBFBF;font-size:13pt" | height="17" valign="bottom" | XF | align="center" valign="bottom" | NEB RCA 3 hrs LP (LLRC***) | align="center" align="center" valign="bottom" | 54.3 | align="center" valign="bottom" | | align="center" align="center" valign="bottom" | 188 | align="center" align="center" valign="bottom" | 875.24 |- style="font-size:13pt" | height="33" valign="bottom" | XA (-) | valign="bottom" | NEB RCA 3 hrs CP (LLRC) - Primer for antisense (-) strand | align="center" align="center" valign="bottom" | 49.2 | align="center" valign="bottom" | | align="center" align="center" valign="bottom" | 188 | align="center" align="center" valign="bottom" | 793.04 |- style="background-color:#BFBFBF;font-size:13pt" | height="17" valign="bottom" | XA-No primer | valign="bottom" | NEB RCA 3 hrs CP (LLRC) No added primer | align="center" align="center" valign="bottom" | 2.73 | align="center" valign="bottom" | | align="center" align="center" valign="bottom" | 188 | align="center" align="center" valign="bottom" | 44 |} [[Category:ComboLock]] [[Category:20170324]]
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