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==Hybridization== '''Sample Matrix''' {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> |- style="background-color:#8DB4E2;font-size:12pt;font-weight:bold" align="center" | width="65" height="30" | Sample | width="65" | [[Daniel:Notebook/HiResChrPaint/2014-1-24#Labeling Results|Cot1-488]] (ug) | width="65" | V6S2-546 (pmol) | width="65" | V6S3-546 (pmol) | width="65" | Cot1 (ug) |- style="font-size:12pt" align="center" |style="font-weight:bold" height="15" | A | align="center" | 0 | 8.7 (20) | align="center" | 0 | 2.5 (2.5) |- style="background-color:#D9D9D9;font-size:12pt" align="center" |style="font-weight:bold" height="15" | B | align="center" | 0 | align="center" | 0 | 7.1 (20) | 2.5 (2.5) |- style="font-size:12pt" align="center" valign="bottom" |style="font-weight:bold" height="15" | C | 5 (0.4) | align="center" | 0 | align="center" | 0 | align="center" | 0 |} #Allow stored samples to warm to room temperature #Incubate in 2X SSCT +50% (v/v) formamide for 2.5 minutes at 92 C in pre-warmed media #Incubate in 2X SSCT + 50% formamide at 60C for 20 minutes #Remove samples and allow to cool to room temperature #Add 25 uL hybridization cocktail to center + 1 uL RNase A #Add 22x22 #1.5 coverslip; seal with rubber cement #Allow the rubber cement to air-dry for 5 minutes at room temperature #Denature for 2.5 minutes at 92C; keep humid #Transfer slides to a humidified chamber and hybridize >24 hrs at 42C ##Continued [[Daniel:Notebook/HiResChrPaint/2014-7-17|7/17/2014]] [[Category:HRCP]] [[Category:20140708]] [[Category:ULYSIS Label]] [[Category:Hybridization]]
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