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==Discussion== First I'll establish the math behind this. The equations are numbered for convenience. [[Image:Equations-20170622.png]] The important equations are '''3''', the idealized y=mx+b with y as number of cycles and x as log(input), and '''4''', which is '''3''' but corrected for the efficiency (e<sub>x</sub>) of reaction explicitly added. Note that efficiency should be between 0 and 1. So using the above equations for sample and control cycling as a function of input. From those suppositions and using the ''controls'', we can calculate the b' constant as 15.737 and m' as 11.138. From those, we can estimate the constants and efficiencies from the ''samples'' trendline: [[Image:Equations2-20170622.png]] So the '''m''' based calculations doesn't make any sense but '''b''' gives an answer of 0.497, which is reasonable. So maybe it's 50% efficient? If so, the following table will dictate how many cycles it would take to amplify given ammounts. Bolded samples are included but from data. {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> |- style="background-color:#92CDDC;font-size:12pt;font-weight:bold" align="center" | width="100" height="28" | Input | width="110" | Cycles |- style="font-size:12pt" align="center" valign="bottom" | height="15" | '''1 pmol''' | align="center" | '''92.5''' |- style="background-color:#BFBFBF;font-size:12pt" align="center" valign="bottom" | height="15" | '''500 nmol''' | align="center" | '''87.5''' |- style="font-size:12pt" align="center" valign="bottom" | height="15" | '''100 nmol''' | align="center" | '''111''' |- style="background-color:#BFBFBF;font-size:12pt" align="center" valign="bottom" | height="15" | 10 nmol | align="center" | 114.0 |- style="font-size:12pt" align="center" valign="bottom" | height="15" | 1 nmol | align="center" | 119.7 |- style="background-color:#BFBFBF;font-size:12pt" align="center" valign="bottom" | height="15" | 100 fmol | align="center" | 125.4 |- style="font-size:12pt" align="center" valign="bottom" | height="15" | 10 fmol | align="center" | 131.1 |- style="background-color:#BFBFBF;font-size:12pt" align="center" valign="bottom" | height="15" | 1 fmol | align="center" | 136.8 |- style="font-size:12pt" align="center" valign="bottom" | height="15" | 100 amol | align="center" | 142.5 |} [[Category:PosSeq]] [[Category:20170621]]
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