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==Discussion== It's not exactly easy to spot unless you have a fancy computer, so here's the gist. The '''blank beads samples''' show that without DNA the DRAQ5 doesn't stain the beads, and there isn't enough autofluorescence at that wavelength to look like much. When you do max projection (which all of these are) and adjust the thresholds to similar values as with the samples, the resulting signal is not next to the dots. The '''dsDNA positive control''' on the other hand, has the signal overlap with the beads, although not on all samples. This makes sense, however, as those beads are generally under packed (50% of theoretical maximum was added), meaning a simple Poissonian predicts that a lot of them will have little signal. Some, however, will and do have the signal. The '''100 pM samples''' have good signal, although I will note that on the samples the beads tend to stick together more often. The '''1 pM''' samples on the other hand, show no real signal. So the 1 pM sample did not have enough amplification. [[Category:20170628]] [[Category:PosSeq]]
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