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===Sectional=== The other image is a large sectional image, and brings up other questions. [[File:20140916_RNAFISH_FOXP2-546_CUX2-647.lif_Production2_VerticalCut_ch00.jpg|center|1200px|Tissue Sectional, vertical, FOXP2-546 (yellow) and CUX2-647 (magenta)]] One thing to note is that the stitching isn't great with the Leica software, since this was supposed to be a straight image. Of important note, however, is that there is no signal whatsoever near the top of this sectional. One thing I have noticed on these long sections is that the z-dimension can change quite a bit over the length of the sample. This may be the cause of the reduced signal, which is otherwise ubiquitous. We have not yet viewed any real patterns/layers in the fluorescence, which is what we're looking for, but I don't think this is layering, I think it is a microscope error. [[Category:RNAFISH]] [[Category:Microscopy]] [[Category:20140829]]
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