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===Question: how to reduce down to a set of 5hmC-relevant sites?=== * We want to validate a small subset of 5hmC relevant sites - utilize Tet protein binding data * Tet protein occupancy have been found for two cell lines (colon cancer and embryonic kidney) TET2 HCT116 - 2,536 significant peaks - 612 peaks overlap with HEK293T - 785 (31.0% overlap with high variance regions) TET2 HEK293T - 4,270 significant peaks - 612 peaks overlap with HCT116 - 3112 (72.9% overlap with high variance regions) TET3 HEK293T - 4,983 significant peaks - 2952 (59.2% overlap with high variance regions) * Since these TET protein occupancy data are cell type specific, we may be limited in what we can study. Perhaps just in colon cancer or kidney * Overlap all three Tet2 occupancy files, found '''13,530''' regions to target.
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