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==Protocol== # Set up 2 tubes for standard and 12 tubes for samples (2x each 1-6) # Label tube lids # Calculate amount of working solution required. # Load in appropriate volumes for each labeled tubes # Vortex for 5 s and spin down all tubes # Incubate for 2 minutes at room temperature # Turn on Qubit Fluorometer # Press HOME and select Quant-iT ssDNA. Press GO to initiate assay # On calibration screen, select Run New Calibration. Press GO Or select use last calibration # Insert standard#1, close lid, press GO # Remove standard#1, insert standard#2, close lid, press GO # Remove standard#2. # Repeat similarly for sample assay tubes. # Record readings given by the fluorometer. # Turn off Qubit Fluorometer after last reading: press HOME, scroll to turn off, then press GO to turn it off.
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