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===NCIH1975 Rolony : 1st try=== *Sample : Glass-bottom MatTek dish β 2 dishes *NCIH1975 P25 (from Illumina) *Process 11/30~12/02 *Image Cy5-1st Rolony detection probe (1uM in 30% Formamide + 2xSSC) ====Result(Preliminary)==== *The number of rolonies were not many, not as dense as that from PGP1F cells. **--> However the rolony/cell is counted ~200 which looks fine. *Cells were not dense, it maybe reason why there is lower density of rolonies, need to wait 2~3 days more to get denser cells. *'''The thickness for z-stack is about 50~60um which is 2~3 times thicker than PGP1F (15~20um).''' **Do I need to analyze 3D-Rolonies along z-stack?... *Further analysis is on going. =====Number of Rolony===== *S1, position 2 *Laser Power = 4% *PMT Gain = 550 --> Count = 6311 *Cells = ~30 *Rolony/Cell = 210.37 *[[Media:NCIH1975-20131202_S1_Step1_Pos2_G550.tif|Raw image of S1, Pos2]] [[File:NCIH1975_prelimResult_S1_Pos2.png|1200px]]
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