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====Result==== *Rolonies from 1000b primer were too many, they are much more than control (random hexamer). *There is a trend, 220b and 300b primer sample don't have many rolonies which are almost similar number that we've seen so far with RAB7A detection. *However 500b sample showed more rolonies, and 1000b sample has too many and too much strong signal. *I'm not sure the signal from 1000b sample is real signal or something other things...because it's too much. *But since I've made 4 wells per each sample, and all 4 wells have the sample result per each primer, so I think these images shows some meaningful results. *Raw images : [[Media:MIP_RTPrimer_RAB7A_C3_Hexamer-1_Step1_Pos1.tif|Control]], [[Media:MIP_RTPrimer_RAB7A_D3_D220-1_Step1_Pos1.tif|D220]], [[Media:MIP_RTPrimer_RAB7A_D7_D300-1_Step1_Pos1.tif|D300]], [[Media:MIP_RTPrimer_RAB7A_E3_D510-1_Step1_Pos1.tif|D510]], [[Media:MIP_RTPrimer_RAB7A_E7_D1000-1_Step1_Pos1.tif|D1000]] *[[File:MIP_RTPrimer_1stRolony_Compare.png|800px]] *1st Rolony Counts [[File:PGP1F_96well_RTpositionTest_1stRolonyCount.png|800px]] *PISA parameters **Gaussian Std : 3 **Gaussian Upper : -2e-4 **area upper: 50 **area lower: 4 **axratio lower: 0.6 **circ upper: 1.6 **circ lower: 0.8 **perim conn: 8 **bkgmult lower: 4
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