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====Result==== *Confocal setting : 20x oil lens --> 580um x 580um view area (1x zoom) *512 x 512 pixel seemed not enough, 1024 x 1024 or 2048 x 2048 would be good for recognizing rolonies **1 pixel size of 512 resolution was 1.14um which is slightly bigger than normal size of rolony (~700nm) **1024 res. --> 1 px = 580um, 2048 res. --> 290um for 1 pixel *I've taken tile scan images --> 8 x 6 tiles, with 19 stacks along z axis. --> Total scan are = 4.64mm x 3.48mm *For tile scan, we used 512x512 resolution, and the total scan took about 35 min. **However, we're going to use 2048 resolution for the next time. *Convert max. projection for image analysis *'''Analyzing images is on going.''' *About 30 cells in one field of view (580um x 580um, 512x512 pixel resolution with 20x obj.) --> Total number of cells = ~ 1440 *But by the temporary analysis, **'''Roughly''', the ratio (total rolonies)/(ACTB target rolonies) in 8x6 tiles = ~ 4900/140000 = ~ 3.5%
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