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===Set up MDA reactions=== *MDA Mix 1ul template with 1ul ALS buffer, RT for 3min Add 2ul NS buffer Add 10ul PNP (Primer-nucleotide-protease) buffer 10x RepliPhi Buffer 1.4ul 1mM N6 primer 1.0ul 25mM dNTP 0.8ul 0.05AU/ml Protease 0.5ul H2O 6.5ul 37C 30min -> 70C 15min -> 4C hold Add 6ul ED (Enzyme-Dye) buffer 10x RepliPhi Buffer 0.6ul 2X SYBR Green I 1.0ul RepliPhi Phi29(100U/ul) 1.0ul H2O 3.4ul Incubate at 30C for 4h on Opticon4, take one read every 6 minutes.
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