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===Results=== *Olympus Imaging 6/19/2015 (Stored in DARTFISH-RNAFISH folder on desktop) **RNAFISH1: Alexa 488 (GFP filter) and Alexa 594 (TXRED filter) **RNAFISH2: Alexa 546 (Cy3 filter) and Alexa 647 (Cy5 filter) *Use [http://rajlab.seas.upenn.edu/StarSearch/launch.html StarSearch from Raj Lab] **Doesn't work in Chrome browser (use IE) **Uses a histogram of pixel intensities and a threshold to determine how many "stars" *Still see a lot more background than example images on [https://plus.google.com/photos/108920949724677333478/albums/5859252817136626497?hl=en&partnerid=gplp0 Raj lab website] **[https://sites.google.com/site/singlemoleculernafish/faq#TOC-Imaging Arjun Raj FAQ for sm RNA FISH says to use cooled CCD camera] ====RNAFISH1==== *Pos1 488-CUX2 (GFP) **Most spots aren't in cell [[File:RNAFISH1_Pos1_GFP_StarSearch.png|450px]] *Pos1 594-KIT (TXRED) **Probably not mRNA signal? [[File:RNAFISH1_Pos1_TXRED_StarSearch.png|450px]] *Pos2 488-CUX2 (GFP) [[File:RNAFISH1_Pos2_GFP_StarSearch.png|450px]] *Pos2 594-KIT (TXRED) [[File:RNAFISH1_Pos2_TXRED_StarSearch.png|450px]] ====RNAFISH2==== *Pos1 546-CUX2 (Cy3) **Focus on dendrites [[File:RNAFISH2_Pos1_Dendrites_Cy3_StarSearch.png|450px]] **Focus on nucleus [[File:RNAFISH2_Pos1_Nucleus_Cy3_StarSearch.png|450px]] *Pos1 647-KIT (Cy5) **Focus on dendrites [[File:RNAFISH2_Pos1_Dendrites_Cy5_StarSearch.png|450px]] **Focus on nucleus [[File:RNAFISH2_Pos1_Nucleus_Cy5_StarSearch.png|450px]] *Pos2 546-CUX2 (Cy3) *Pos2 647-KIT (Cy5)
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