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=== Part II === * In the next experiment, I plan to test the amplification by the second set primers with the DNA amplified by different concentration of randomly tagging primers and also vary the amount of USER. From the previous experiment (2011_09_02), even we can not see the amplification by the lowest concentration primers (0.02uM), but I still test this condition. The reason was that in the previous experiment the concentration of primers was kept very low at 0.02uM for the whole procedure. But in the actual experiment, the second set primers will be added after the 2nd cycle of amplification and the concentration is much higher at 0.2uM. We might see better amplification in this condition when compare to the previous experiment. * I also vary the volume of USER (1, 2 & 5ul) even in the previous experiment I can not see the amplification after USER digestion. This just wants to confirm again that USER can completely digest the uracil containing primers and 1ul of USER is good enough.
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