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==== Qubit dsDNA HS assay result ==== {| {{table}} class = wikitable | align="center" style="width:120px;background:#f0f0f0;"|'''Sample''' | align="center" style="width:80px;background:#f0f0f0;"|'''Conc. In the Qubit''' | align="center" style="width:60px;background:#f0f0f0;"|'''Unit''' | align="center" style="width:60px;background:#f0f0f0;"|'''uL used''' | align="center" style="width:70px;background:#f0f0f0;"|'''Dilution''' | align="center" style="width:70px;background:#f0f0f0;"|'''Sample conc. (ng/ul)''' | align="center" style="width:70px;background:#f0f0f0;"|'''Volume (ul)''' | align="center" style="width:70px;background:#f0f0f0;"|'''Yield in 400ul (ug)''' |- | 6-T-1||434||ng/mL||2||100||43.40||400.00||17.36 |- | 6-T-2||316||ng/mL||2||100||31.60||400.00||12.64 |- | 6-T-3||315||ng/mL||2||100||31.50||400.00||12.60 |- | 6-T-4||417||ng/mL||2||100||41.70||400.00||16.68 |- | 6-T-5||305||ng/mL||2||100||30.50||400.00||12.20 |- | 7-T-1||307||ng/mL||2||100||30.70||400.00||12.28 |- | 7-T-2||446||ng/mL||2||100||44.60||400.00||17.84 |- | 7-T-3||359||ng/mL||2||100||35.90||400.00||14.36 |- | 7-T-4||338||ng/mL||2||100||33.80||400.00||13.52 |- | 7-T-5||204||ng/mL||2||100||20.40||400.00||8.16 |} * I also measured with ND to determined DNA extraction quality and compare measurement by Qubit and ND {| {{table}} class = wikitable | align="center" style="width:120px;background:#f0f0f0;"|'''Sample ID''' | align="center" style="width:80px;background:#f0f0f0;"|'''ND conc. (ng/ul)''' | align="center" style="width:80px;background:#f0f0f0;"|'''260/280''' | align="center" style="width:80px;background:#f0f0f0;"|'''260/230''' |- | 6-T-1||57.51||2||1.67 |- | 6-T-2||84.83||1.99||1.54 |- | 6-T-3||42.86||1.84||1.04 |- | 6-T-4||55.42||1.69||0.96 |- | 6-T-5||36.96||1.93||1.26 |- | 7-T-1||39.44||1.51||0.89 |- | 7-T-2||66.63||1.78||1.36 |- | 7-T-3||62.39||1.83||1.37 |- | 7-T-4||49.6||1.91||1.53 |- | 7-T-5||28.74||1.68||1.07 |} * The concentrations from both Qubit and ND are not too much different. However the ratio of 260/230 is quite low in most samples. I am not sure if this because I reloaded column with double amount of lysed cells. * The total of gDNA extracted from tumor tissue is not very high compare to the size of tissue. I think the majority of the tissue in more fibers and the components of connective tissue.
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