Editing
Noi:Noi' Reagents
(section)
Jump to navigation
Jump to search
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
== WGBS libraries of normal and cancer patients == * Please note that there are two batches of WGBS libraries of ctDNA from cancer patients. The first batch I generated using the protocol similar to RRBS but skipped DNA digestion and used 1ng of ctDNA as starting materials as described in XXXX. This batch of WGBS libraries was used for the first Nimblegen SeqCap hybridization capture experiment on XXXX, I have them all (24 libraries in PCR rack that contain in ''Noi Losen Stuffs 2'' at -20C as shown I'n picture above. * '''The second batch of WGBS from ctDNA of cancer patients''' were generated using '''KAPA Hyper Prep Kit''' with more input of starting material (in the range of 1.5-20ng). They were generated on XXXX and should be located in the PCR rack with cover (on the left of image below). [[File:NP_photo 8-e.JPG|450px]] * I also have WGBS of cfDNA from normal control generated on XXXX in the same box of the first batch of WGBS libraries of cancer patients (Noi Losen Stuffs 2 box). However, I have performed re-amplification of these normal samples and stored in different location that will be described below. I may not suggest to use all WGBS libraries in Noi Losen Stuffs 2 box. * There are 24 WGBS of normal control re-amplified on XXXX, However, these WGBS are generated by the method similar to RRBS without DNA digestion or any fragmentation. I did not generate more WGBS libraries of normal control using KAPA Hyper Prep Kit as they showed good result either in BSPP capture and SeqCap hybridization capture. Dr. Zhang suggested to generate more WGBS libraries only for those cancer samples which have poor results. ==== Final conc. of WGBS libraries ==== ==== WGBS libraries of ctDNA from cancer patients ==== (The tube should be labeled ~2014-12-13) :{| {{table}} border = 1 | align="center" style="width:100px;background:#f0f0f0;"|'''Sample''' | align="center" style="width:80px;background:#f0f0f0;"|'''Conc. (ng/ul)''' | align="center" style="width:80px;background:#f0f0f0;"|'''TruSeq Indx''' |- | PCP-9||26.13||8 |- | PCP-4||21.73||3 |- | PCP-8||19.07||7 |- | PCP-6||20.13||5 |- | PCP-7||22.80||6 |- | PCP-2||22.13||1 |- | PCP-3||21.47||2 |- | PCP-5||21.47||4 |- | 6P-1||26.00||9 |- | 6P-8||24.80||14 |- | 6P-10||22.27||16 |- | 6P-9||20.27||15 |- | 6P-5||24.67||13 |- | 6P-2||19.60||10 |- | 6P-3||19.87||11 |- | 6P-4||19.20||12 |- | 7P-8||22.80||25 |- | 7P-3||19.07||20 |- | 7P-10||25.33||27 |- | 7P-7||29.60||23 |- | 7P-6||26.93||22 |- | 7P-1||14.67||18 |- | 7P-2||19.73||19 |- | 7P-5||18.00||21 |} ==== WGBS libraries of cfDNA from normal controls ==== (The tube should be labeled ~2014-12-16) :{| {{table}} border = 1 | align="center" style="width:100px;background:#f0f0f0;"|'''Sample''' | align="center" style="width:80px;background:#f0f0f0;"|'''Conc. (ng/ul)''' | align="center" style="width:80px;background:#f0f0f0;"|'''TruSeq Indx''' |- | NC-1||13.73||1 |- | NC-2||16.00||2 |- | NC-3||16.93||4 |- | NC-5||13.07||5 |- | NC-6||15.33||6 |- | NC-7||13.73||7 |- | NC-8||15.87||8 |- | NC-9||16.53||10 |- | NC-12||14.80||13 |- | NC-13||13.19||14 |- | NC-14||14.93||15 |- | NC-15||13.60||16 |- | NC-16||13.87||27 |- | NC-17||13.47||18 |- | NC-18||14.67||19 |- | NC-19||13.47||20 |- | NC-20||12.79||11 |- | NC-21||12.31||12 |- | NC-22||16.00||21 |- | NC-23||13.60||22 |- | NC-24||11.97||3 |- | NC-27||14.67||9 |- | NC-29||12.96||23 |- | NC-30||15.47||25 |}
Summary:
Please note that all contributions to ZhangLabWiki may be edited, altered, or removed by other contributors. If you do not want your writing to be edited mercilessly, then do not submit it here.
You are also promising us that you wrote this yourself, or copied it from a public domain or similar free resource (see
ZhangLabWiki:Copyrights
for details).
Do not submit copyrighted work without permission!
Cancel
Editing help
(opens in new window)
Navigation menu
Personal tools
Not logged in
Talk
Contributions
Create account
Log in
Namespaces
Page
Discussion
English
Views
Read
Edit
View history
More
Search
Navigation
Main Page
Current events
Recent changes
Random page
Investigators
Matt Cai
Song Chen
Eric Chu
Dinh Diep
Elizabeth Duong
Shicheng Guo
Alan Fung
Daniel Jacobsen
Blue Lake
Huy Lam
Alice Li
Andrew Richards
Brandon Sos
Chris Wei
Yan Wu
Kun Zhang
Tools
What links here
Related changes
Special pages
Page information