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==Size selection and re-amplification== * Directly load combine 1-2, 3-4, 5-6 and 7-8 for size selection * Gel was pooled into 4 tubes: 100pg: >300, <300 10pg: >300, <300 * EtOH precipitation, resuspend in 11ul, 1ul for Qubit #1.10pg,<300bp 2.60ng/ul 26ng #2.10pg,>300bp 1.45ng/ul 14.5ng #3.100pg,<300bp 10.7ng/ul 107ng #4.100pg,>300bp 7.41ng/ul 74.1ng * Post-size amplification: Double confirm dsDNA with selected size range Produce enough DNA for DSN treatment Option1: mix <300bp and >300bp )(10pg and 100pg) for DSN Option2: reAmp for a few cycles individually Take option 2, reAmp #1 and #2 for 3 cycles (goes with 4 cycles for safe), #3 and #4 for 1 cycle in 100ul PCR [[File:12.18.12_reAmp.1-2.jpg|400px]][[File:12.18.12_reAmp_gel.jpg|400px]] * beads purification, Qubit measurement #1.10pg,<300bp 13.3ng/ul 133ng #2.10pg,>300bp 13.1ng/ul 131ng #3.100pg,<300bp 4.65ng/ul(?) 93ng(?) #4.100pg,>300bp 8.82ng/ul 176ng
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