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===AMpure beads purification (multi-channel pipettor)=== Set up: UV-ed Solution Basin - for 70% EtOH (6mL) UV-ed 8-well PCR tube strip (high-profile, Biopioner) UV-ed 8-well PCR tube strip w/ individual cap (high-profile, Eppendorf) -> for collecting purified products *Load 100uL libraries into PCR tubes. *Load 1V AMpure beads (100uL). Mix by repeat pipetting 20 times(200uL, multi-channel pipettor). Incubate at RT for 5min. *Relocate onto magnetic board. Incubate at RT for 2min. Remove the supernatant. *Add 200uL 70% EtOH into the PCR tubes(no pipetting). Incubate at RT for 1min. Remove the supernatant. *Repeat the EtOH wash again. *Use 10p tips to remove the remained EtOH. *Air dry the the EtOH for ONLY 2min. During the air-drying, relocate PCR tubes to 96-well rack. *Add 20uL pre-warmed RT-PCR grade H2O. Mix by repeat pipetting 20times('''WASH DOWN AMPURE BEADS BY PIPETTING AT DIFFERET DIRECTION'''). Incubate at RT for 5min. *Relocate PCR tubes on the magnetic board. Incubate at RT for 2min. *Carefully transfer the supernatant(eluted DNA) into a fresh PCR tube strip('''THE REMAINED MAGNETIC BEADS WILL AFFECT NANODROP READINGS''').
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