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==Plotting== *Since the bin number data is not continuous (the bin number containing no reads will not be reported in previous step). *I need to create a bin-ladder (from bin 0 to 463) and pool my data set with bin-ladder by matching bin number. **Without doing this step will cause Excel draw the plot with discontinuous bin interval (since Excel regard bin number as a new data set but not X-axis). *I pooled the data set using Excel Add-Ins function "Join (merge)Talbes"(from DigDB). *Take the log10 value of total reads for Y-axis. *'''IMPORTANT:''' Since log10 value can't be taken from no read (count=0/10,000bp), I manually assign the value of '''log10 of "no read data"''' as '''"-1"'''. **The reason to do so is that there are lots of bins containing "0 read" exclusively in long-amplified SAGs' data sets. *'''X-Y scatter plot analysis:''' **Purpose: to compare if libraries made from the same MDA amplicon resource (e.g. ID1-ID3) behave more similar than libraries made from different MDA amplicons (e.g. ID1-ID2).
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