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==Procedure== *Sample preparation **Dilute 1ug 1ug of E.coli MDA amplicons into 100uL total volume with H2O. Transfer into a 1.5-mL microcentrifuge tube. **Prepare another tube with 100uL H2O for balancing. ===Operation manual=== *Hook up H20-tubes of cooling system. Release the white clipper. *Fill up H2O(tap H2O is acceptable) in the cup-horn to match the yellow tags *Turn on the switch of the cooling system. *Cooling system setting: Pump speed: "50", Desired temp "4C". *Turn on the switch of main controller. *Program setting: **Press "No" for not using micro-tip. **Press "program" to set up new conditions. **Tested condition: Pulse on(1min), Pulse(10sec), Output power(5), Temp monitoring(No). Total time(30min). *Press "start" to start the shearing. *Press "pause" to pause the shearing and aliqate sheared samples for validation. *After using, use the opposite order of operation procedures to turn off the machine. **Press "Stop" to stop shearing. **Turn off the main controller. **Turn down pump speed to "0" and turn off the switch. **For the cooling system, disconnect the H2O-tubes. Drain the H2O into the beaker. **Wipe out spilled H2O. *Technical support info: 800-323-4340 or 203-426-0101
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