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====Annealing Padlock Probes==== #Add 100ul 2uM ppMALAT1 in 2X SSC @45C for 1hour, 4 hour and 24 hours starts at 3:15pm #Wash 2X with 2X SSC #Prepare dye for 3 scans (30ul 20X SSC, 90ul 100% formamide, 177ul H2O, 3ul Dye) #Preheat dye at 75C for 5 mins #Add 100ul 1uM dcProbe2RevComp-Cy3 in 2X SSC + 30% formamide #Incubate at RT for 10 mins #Wash 2 2X SSC twice
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