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===Purge bubbles from flow cell=== *Open up the middle panel on the cytometer, locate the flow cell (a small window with fluid in it) *Move the side arm to the side *Remove tube containing MilliQ H2O form the sample injection port (SIP) *Turn knob on the control panel to <Drain> in order to drain the flow cell (Notice the fluid level drops) *Once the flow cell is drained, turn the knob to <Fill> (Notice the fluid level rises) *Repeat the drain and fill procedure 3 times or until no bubbles are seen on the wall of the flow cell *Leave the control knob on <Fill> *Close the middle panel
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