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===Digestion with MmeI=== (dUTP 250uM,167.3ng/ul of product will use 6ul of DNA along with 2ul of 1mM SAM and 8ul of 2U/ul MmeI) First attempt: Total 20ul tube 1(257.1ng/ul) 4ul tube 2 (194.6ng/ul) 6ul 10X NEBuffer 4 2ul 10X NEBuffer 4 2ul 1mM SAM(fresh) 2ul 1mM SAM(fresh) 2ul 2U/ul Mme I 8ul 2U/ul Mme I 8ul ddH2O 4ul ddH2O 2ul Second attempt: Total 40ul DNA 13ul 10X NEBuffer 4 4ul 1mM SAM(fresh) 6ul 2U/ul Mme I 8ul ddH2O 9ul 1mM SAM: 32mM SAM 1ul + 31ul ddH2O. 37C 2h MinElute column purify. Elute in 11ul EB. TBE gel result: [[Image:ZhangLab_2 2009-03-14 16hr 19min - TBE.jpg]] [[Image:ZhangLab_2 2009-03-17 10hr 59min-TBU.jpg]]
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