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===results=== *RNA quantification results {| {{table}} | align="center" style="background:#f0f0f0;"|'''''' | align="center" style="background:#f0f0f0;"|'''intensity''' | align="center" style="background:#f0f0f0;"|'''ng/ul''' | align="center" style="background:#f0f0f0;"|'''''' |- | ||2612.720078||5|| |- | ||1701.430051||2.5||in 10 uL |- | Index 49, 1. RNase III, 1000 NPC cells||9239.960275||23.18093033||231.8093033 |- | Index 50, 2. RNase III, 1000 NPC cells||8398.20025||20.87167641||208.7167641 |- | Index 51, 3. RNase III, 1000 NPC cells||9152.810273||22.94184617||229.4184617 |- | Index 52, 4. RNase III, 1000 NPC cells||13081.12039||33.71862963||337.1862963 |- | Index 53, 5. RNase III, 3000 NPC cells||10821.08032||27.51851771||275.1851771 |- | Index 54, 6. Rnase III, 6 ng pure NPC DNA control||50108.68149||135.2986975||1352.986975 |- | Index 55, 7. Rnase III, 6 ng pure Jurkat DNA control||138791.8341||378.5888411||3785.888411 |- | Index 56, 8. RNase III, 1000 cells lysed without transposome complex IVT||2492.030074||4.668903423||46.68903423 |- | Index 57, 9. RNase III, Nuclease free H20 only IVT||1701.540051||2.50030177||25.0030177 |- | |} *RNA after ~16.5 hours IVT, run on TBU gel *can see that it did not really work. washed cells in plate twice before transposition and probably lost them (samples 1,2). washed the vial of cells twice (samples 3,4,5) and maybe lost them there too. NTCs worked. *will not continue experiment. [[File:ZhangLab 2 2013-08-01 15hr 31min-labeled.jpg|600px]]
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