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===conclusions=== *'''Overall will repeat to see if RNA yields increase. Will also try EtOH preciptation again with Guanidine HCl to denature proteins before purification.''' *When pooling will now try using guanidine HCl to remove proteins. Previously 1000 cell samples were not working, maybe since proteins are still bound and/or are not removed efficiently by AMPURe beads alone. *Total amout of RNA generated is lower, however it could be the gel since the positive control is lower *Since normal process did not work, it could be the protease being added is not working. can try ordering a new protease or using a different one to use the normal protocol without cleaning.
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