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====Prepping cells==== *Thaw cells and really gently pipette nuclei with p20 (repeat pipette ~8 times) *Use cell counter (if this is a new sort with unknown concentration of cells) = 8ul loaded onto haemocytometer (in the end, we want to load 600 cells onto the chip) **Double check under the microscope that cells are intact in the haemocytometer. **Normally would put in double amount/volume needed because if not all channels are filled/very low number of them are, we can just put the chip back on the C1 and press load again **Make sure not clumping in haemocytometer in microscope. If the cells are clumped, just mix, let clumps settle to the bottem of the tube, then take only the top layer out to load *'''Keep nuclei on ice until you have to use them'''
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