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Chromosome Separation Attempt 2 (2/25/10)
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==Observations== Nearly the exact same procedure was followed as in attempt 1, this time allowing for two different mixing techniques. Yet the result was the same: indecipherable masses not at all resembling those of typical chromosomes. It appears that this problem traces back to the cell cultures and the inability of the colcemid to fix the cells in the metaphase state. There are two possible reasons for this: 1) The cells are not growing well and are therefore unable to reach metaphase or 2) the colcemid is failing to work. To fix any problems with cell growth we must re-culture a new cell line and be sure to maintain it properly. If the colcemid is not working, it is either because the incubation time is inaccurate or that the chemical itself is failing to work. Since other groups have been able to get results using colcemid, this leads me to believe that the colcemid does indeed function, but that we are either not incubating the cells in the colcemid for the proper amount of time, or - more likely - the cells are poorly maintained and are therefore not growing properly.
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