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===Discussion=== Well, this is a mixed success. The 100 amol sample (1E7 molecules) has 231bp product in all three lanes, and the 10 amol samples (1E6) have signal in 1, kinda 2 lanes. This is a 100-1000 fold signal increase, which is great. There are however, still prevalent primer dimers at ~100bp that are present even in the NTC lane. Addressing these primer dimers will probably increase signal to an even higher level. As it stands 1E7 is approximately 1000 copies of an mRNA (or protein) in an experiment with 10,000 cells. 1E6 is a 100 copy mRNA/protein. [[Category:ComboLock]] [[Category:20160930]]
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