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==Protocol (Part 2)== <ol start="5"> <li>TBU gel</li> <ol type="A"> <li>Prerun gel for 15 minutes at 250V</li> <li>Mix 9 uL TBE, 10 uL 2X Buffer, 1 uL sample for 10 bp ladder, C1-C2 amplicon, and C3-C2 amplicon</li> <li>Remove urea from wells by pipetting up and down with a 200 uL pipette</li> <li>Add 20 uL to each lane</li> <li>Run gel for 25 min at 250V</li> <li>Stain with 3 uL SYBR Gold for 3 min</li> <li>Rinse and image (see gallery below)</li> </ol></ol> Results are inconclusive, I'm going to try a TBE gel with the 25bp ladder, which is generally easier to read than the 10bp ladder <ol start="6"> <li>TBE Gel</li> <ol type="A"> <li>Mix 36 uL TBE and 8 uL 6x Gel Loading dye</li> <li>Aliquot 10 uL for each sample to parafilm</li> <li>Add 1 uL of sample/ladder to the drops</li> <li>Mix with the loading pipette and add 10 uL to each lane</li> <li>Run for 25 min at 250V</li> <li>Stain with 3 uL SYBR Gold for 3 min</li> <li>Rinse and image (see gallery below)</li> </ol></ol> ===Gel Imaging Results=== <gallery perrow=2 heights=300px widths=300px> File:2016-08-16-PosControlAmplicon-TBU.png|TBU Gel Image File:2016-08-16-PosControlAmplicon-TBE.png|TBE Gel Image </gallery>
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