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===Discussion=== At first I was a bit confused by these results, especially the pie charts. There are now more zeros and over-amplifications present, although there are fewer reads used. But I remembered I used the phred 64 flag, which is more stringent, so that probably threw out some data. Since the errors were probably only present one or a few times, they wouldn't have ended up in the zeros category (which had to be equal to zero) or in the over-amplification category (which required ~1000 reads), so they would have been in the normal read count category, which would have reduced the number of normal reads, decreasing normal read percent and increasing the other two.
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