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Dinh:Protocols/Probes Prep Oct6
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==Step 4: Purification with QiaQuick Columns== (Optional) 1ul sample with 4ul dH20 for PAGE 1. Add 5x PBI buffer (790 ul) 2. Mix well 3. Load equally to 2 columns 4. Spin at 14000 rpm->1min 5. Empty collection tubes 6. Add 750 ul wash buffer (PE) 7. Spin at 14000rpm->1min 8. Empty collection tubes & spin again for 2 min 9. Air dry for 15 min 10. Transfer to labeled 1.6ml tubes 11. Add 30 ul ddH20 to each center of each column 12. Wait 1 min 13. Spin at 14000rpm->1 min 14. Pipette remaining dH20 through the center and spin again at 14000rpm->1min 15. Combine 2x30ul into 1 PCR tube. 16. Leave in 4C overnight.
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