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Hosuk:FISSEQ Primer information
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===Detection Probes=== *FISSEQ_Adpt : Cy3 labeled probe for 1st Rolony detection **/5Cy3/TCTCGGGAACGCTGAAGA *FISSEQ_AdptCy5 : Cy5 labeled probe for 1st Rolony detection **/5Cy5/TCTCGGGAACGCTGAAGA *ACTB_1stRolonyFISH : ATTO488 labeled probe for ACTB on 1st Rolony **GGCAAAGGCGAGGCT/3ATTO488N/ *RAB7A_1stRolonyFISH : ATTO550 labeled probe for RAB7A on 1st Rolony **GAGGAGACTAAACGGAGGACA/3ATTO550N/ *MALAT1_1stRolonyFISH : Cy3 labeled probe for MALAT1 on 1st Rolony **/5Cy3/AATGGAGGTATGACATATAATCT *Shifted FISSEQ_2ndRCAprimerDye : 2nd Rolony from all padlock probes **GGATATCGGGAAGCTGAA/3ATTO488N/ *dcProbe0-Cy3 : for 2nd Rolony of ppACTB **Cy3-ACGTATCGGTAGTCGCAACGCA *dcProbe1-Cy3 : for 2nd Rolony of ppRAB7A **Cy3-GTCTTGCGTGCGATACGGAGTA *dcProbe2-Cy3 : for 2nd Rolony of ppMALAT1 **Cy3-TCTACTTCGTCGCGTCAGACCA *dcProbe3-Cy3 : for 2nd Rolony **Cy3-CCGTTTGATCGTTCGACCGAGA *dcProbe4-Cy3 : for 2nd Rolony **Cy3-CTACCATTCGCGTGCGATTCCA *RT-dT50 : Cy3 labeled probe for mRNA detection **/5Cy3/TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT *RT-Adapt : Cy5 labeled probe for cDNA after RT **/5Cy5/TCTTCAGCGTTCCCTAGA *dcProbe2RevComp-Cy3 : ATTO488 labeled probe for detecting the reverse complementary sequence of dcProbe2 used for detecting ppMALAT1 and any other padlock probe before RCA with dcProbe2 in barcode **TGGTCTGACGCGACGAAGTAGA/3ATTO488N/
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