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===How to estimate cell/chr density using fluorescent microscope=== *Load cell/chr solution to a teflon coated slide, cover with a coverslip, then count the number of features per image. *We need to estimate the volume of solution under the imaging area. **The Hamamatsu Orca R2 camera has 1.3M pixels, and each pixel is 6.45 micron in size. Since the sensor is (almost) a square, the width should be ~1200 pixels on each sides, or 1200 x 6.45 um = 7.74 mm. Under a 20x objective, each image corresponds to a 0.39mm x 0.39mm square area, or 0.19mm x 0.19mm under a 40x objective. **To estimate the height, roughly 5ul of solution can fill a well of ~12mm diameter. So the height is 5ul/(3.14x6mm^2)= 44 um. 20x objective 40x objective Area 0.39mm x 0.39mm 0.19mm x 0.19mm Volume 6.62uL 1.66nL
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