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==Results== *The concentration of HMW gDNA seems to be under-estimated by 10-fold. Amplification curves came up faster than those from pure gDNA. *The concentration of chrL seems to be slightly over-estimated (~2-fold). Amplicons 2E&2F were likely amplified from a subset of chromosomes. *The concentration of chrS seems to be under-estimated (~5-fold). Amplicons 1F/1G were likely amplified from a subset of chromosomes. [[Image:2010-08-19-gDNA-control-MDA.png|300px]][[Image:2010-08-19-HsChr-MDA.png|300px]] *What to do next? Purify 1A/1F/1G/2E/2F with Qiaquick columns, shear and make bar-coded Illumina libraries. Sequence all four libraries in one lane (could be mixed with other compatible libraries). Yield: 1A: 39ng/ul x 40ul = 1.56ug 1F: 29ng/ul x 40ul = 1.16ug 1G: 24ng/ul x 40ul = 960ng 2E: 15ng/ul x 40ul = 600ng 2F: 28ng/ul x 40ul = 1.12ug
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