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===Zymo DNA Clean & Concentrator 100=== *Tried using reservoir & multi-channel pipette removing product from 6 strips of pcr tubes but spilled ~1ml (product+binding buffer) pouring from reservoir into 50ml tubes *Purify 10ml of PCR product with 1 Zymo-spin V Column/Reservoir **Add 50ml DNA Binding Buffer to 10ml product (Use 2 50ml tubes) *Elute with 400ul H2O **Somehow final volume is greater than 400ul *Measured concentration with Nanodrop: ~410ul of V4 probes: 76.1 ng/ul => 31.2ug ====Potential Reasons for Low Yield==== *Second time I got 30ug when normal for EtOH precipitation method is 50ug *Possible reasons: **Potentially the vacuum is not strong enough to pull all solution through? (even though it's slow this is not likely) **Losing solution on the sides of the 50ml tubes used to mix binding buffer + amplicon (definitely happening but shouldn't be 40% of amplicon) **400ul elution volume still too much to allow all elution buffer to get out of column membrane when centrifuged *From Alan: Try heating up elution buffer or incubating the column with elution buffer at 37C for 5min
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