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===Comparison=== [[File:20150712_With_vs_Without_Suppressorv2_Scatterplot.PNG|650px]] *Orange line is y=x *Blue line is trendline *The 4 genes suppressed by v2 design are labeled **Being left of y=x indicates suppressor oligos decreased the number of padlock probes capturing the gene/target ====Conclusions==== *Suppressorv2 is effective at decreasing capture counts of most captured genes *Good correlation between dT and Ran primer counts **6 genes did not appear in dT dataset but could be due to lower sequencing depth of that library (those genes should have poly-A tail) *NegCtrl correlates better with gDNA than RNA so probably a little DNA contamination in BA8 sample **Low enough that probably negligible ***eg. the cDNA Ran primer library was taken out of PCR before the NegCtrl curve started to go up [[Media:20150712_With_vs_Without_Suppressorv2.xlsx | Excel analysis]]
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