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===Lambda Exo=== *Divide into 8 pcr tubes of 100ul (ideally should be <10ug each) {| {{table}} | align="center" style="background:#f0f0f0;"|'''Components''' | align="center" style="background:#f0f0f0;"|'''Volume''' |- | Amplicon||50 |- | 10X Lambda Exo Buffer||10 |- | Lambda Exonuclease||10 |- | H2O||30 |- | Total||100 |} *Incubated at 37C for 2hr (normally 1hr) *Purified with 8 Zymo ssDNA/RNA columns **Dropped one column so only 7 *Eluted with 40ul each *Recombined and measured ssDNA with Nanodrop: **118.7ng/ul x 280ul = 33.24ug
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