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==Strip Second Strand== #Remove SecureSeal and put slide in glass Coplin jar of Hexane for 5min #Remove coverslip, wave slide to evaporate residual hexane, and scrape off residual adhesive #Make Wash 1E: 10mM Tris + 50mM KCl + 2mM EDTA + 0.01% TX-100 #Shake slide in plastic box of Wash 1E twice for 4min #Apply blue FrameSeal chamber #*Hoping to use smaller volumes of Wash 1E #Add ~800ul Wash 1E and then aspirate 2 times
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