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Matthew Cai:LabNotes/Probe Production and Capture
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===Nicking endonuclease digestion=== * Incubate 4ug of amplified amplicon with 10ul of Nt.AlwI (10U/ul) in total volume 200ul at 37C for 1hr (4x reactions?) * Heat inactivate enzyme at 80C for 20 min * Add 10ul Nb.BsrDI (10U/ul) at 65C for 1 hr * Purify with Qiaquick colums, elute with 50ul EB buffer each * Measure the conc. of nicked DNA with Nanodrop:__
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