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=== Bisulfite conversion === :- Perform bisulfite conversion by EZ-Lightning DNA Methylation kit using instructions provided by manufacturer without using tRNA as carrier. :- Elute with 12ul of Elution Buffer. The left over volume of DNA is ~10ul. :- Continue to the the steps of DNA SMART ChIP-Seq protocol <ve> '''<u> Note from DNA SMART ChIP-Seq kit</u>'''<br> '''Sample Requirements'''<br> This kit can generate sequencing libraries from 100 pgβ10 ng of ssDNA or dsDNA up to 2 kb in length, but it has been optimized specifically for ChIP DNA with an average size of 200β400 bp. If your starting material is significantly larger, you may need to increase the number of PCR cycles to obtain sufficient yield after size selection Starting material should be in 1β20 Β΅l of an alkaline, low ionic buffer such as the DNA Dilution Buffer (5 mM Tris-HCl pH 8.5) provided, or water. The optimal pH range of samples is 7.5β8.5. Avoid EDTA-containing buffers <br>
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