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== Expansion PCR (Quick test, round2) == * As discussed above, I did a quick run (10ul reaction) with following condition ** Subset A: 100nM template, 15 and 16 cycles ** Subset B: 150nM template, 16 and 17 cycles ** Subset C: 150nM and 200nM. 18 cycles * This time, I used a white real-time PCR tube, so the fluorescent intensity is higher than using a clear tube in the first test. [[File:2014-08-21_test_ExpPCR-90k_15x.PNG| 500px]] [[File:2014-08-21_test_ExpPCR-90k_16x.PNG| 500px]] [[File:2014-08-21_test_ExpPCR-90k_17x.PNG| 500px]] [[File:2014-08-21_test_ExpPCR-90k_18x.PNG| 500px]] * Loaded 5ul of amplicons from each subset at different PCR conditions and also re-loaded amplicons from the 1st test in 6% TBE gel [[File:ZhangLab_2 2014-08-21 18hr 58min_90k-testExpPCR-r2.jpg|500px]]
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