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==== FFPE and Frozen Matched Pair Genomic DNA:Human Tumor Tissue: Colon ==== *<u>'''2014-05-23:'''</u> Received two tubes of FFPE and frozen matched pair gDNA from Biochain ** Sample info. *** Catalog number: D8235090-FP, lot. B609045 *** Tube1: Matched pair - gDNA - human primary tissue: colon. Conc.: 0.58ug/ul or 580ng/ul, amount: 2ug --> expected volume ~3.44ul *** Tube2: Matched pair - gDNA - human primary tissue: colon. Conc.: 0.076ug/ul or 76ng/ul, amount: 2ug --> expected volumed ~26.31ul **** (2014-06-01) I added TE buffer to make conc. to 50ng/ul (total volume 40ul) based on original conc. and re-measured conc. with Qubit dsDNA HS assay again (1ul). ::{| {{table}} class = wikitable | align="center" style="width:120px;background:#f0f0f0;"|'''Sample''' | align="center" style="width:100px;background:#f0f0f0;"|'''Original conc. (ng/ul)''' | align="center" style="width:100px;background:#f0f0f0;"|'''Estimate volume (ul)''' | align="center" style="width:100px;background:#f0f0f0;"|'''Dilute to 50ng/ul (total volume)''' | align="center" style="width:100px;background:#f0f0f0;"|'''Add TE buffer (ul)''' | align="center" style="width:100px;background:#f0f0f0;"|'''Qubit conc. (ng/ul)''' |- | CTT-Frozen||580.00||3.45||40.00||36.55||52.3 |- | CTT-FFPE||76.00||26.32||40.00||13.68||64.8 |} ::- '''CTT = colon tumor tissue''' ** RRBS library preparation of 5ng input DNA: [[http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2014-6-3]] ** RRBS library preparation of 100ng input DNA: [[http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2014-6-13]]<br> <br>
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