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==DSN treatment== * Reference: lab note on 11.7.12 [http://genome-tech.ucsd.edu/LabNotes/index.php/Rui:LabNotes/SingleCell/2012-11-7] * it seems after 1.5hr treatment, all smears shift down below 400bp. Therefore, for the >300 fraction, I decided to do 1hr DSN treatment; 2.5hrs for <300 fraction. * DSN treatment had been used for three times: 10.18.12 (flat after 1hr); 11.6.12 (reasonable delay); 11.17.12 (no much delay) * However, in term of protocol, 10.18.12 is the right one; 11.6.12 has two major issues - [NaCl], 2X instead of 10X DSN buffer was used due to problem in 10X. All of them leads to greatly reduced (10X more) DSN efficiency... * Anyway, decide to follow the 10.18.12's procedure. ===Test=== [[File:12.17.12_preDSN.jpg|400px]][[File:12.17.12_postDSN.jpg|400px]] [[File:12.17.12_DSN_gel.jpg|400px]] ===postDSN amplification=== [[File:12.17.12_postDSN_all.jpg|400px]][[File:12.17.12_postDSN_gel.jpg|400px]] * Beads purification, 0.8X * elute in 11ul, 1ul for gel
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