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==Discussion== *We accidently using PCR program with Tm=52 without checking the primers' desired Tm from previous study. However the amplication were still successful and no obvious un-specific amplification was found. It is possible that the lower Tm might be more suitable for amplyfying on MDA templates. *Optimal condition for Bac MDA amplicon validation: Tm=52C and 35 cycles of PCR amplification, run at 10 uL reaction, using regular Taq enzyme. **94C 2m -> (94C 40s -> 52C 40s -> 72C 1m) x 35 -> 72C 10m -> 15C forever *Both E.coli gDNA and MDA template (from whole single E.coli) came out pretty high representation rate for the eight genes (primers) we tested. The high representation rate might be due to the small size of E.coli genome compared to mammalian cells, or the underestimated the bacteria number in a MDA reaction.
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