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==Results== *PCR program (G-PCR): Step1 94C 2min Step2 94C 40sec Step3 50C-70C 40sec Step4 72C 1min Step5 Goto step2 for 34 cycles Step6 72C 10min Step7 End [[File:sam041610-gradient PCR on short -PCR primer.jpg|480px]] [[File:sam041610-gradient PCR on very-short -PCR primer.jpg|500px]] C.3~C.10 (column 3 ~ column 10) *The expected (designed) Annealing temp: **'''short-PCR primeris 64C''' **'''very-short-PCR primer is 59C''' *The results suggest that not obvious difference in product using different Annealing temperature. **'''I will just keep using the original Tm for these two primers'''.
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