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==Results== *I also did 16 samples MEGAN analysis using 10,000 reads to check if there any bias due to the sampling size (10,000 reads vs 1,000 reads). The results suggest that the bias variation is within 1~3%. Sampling 1,000 reads or 10,000 drew very similar taxonomic conclusions. **I can't test more than 10,000 reads because the limit the BLASTN efficiency. '''Previous bowtie mapping results summary''' [[File:050311_Ecoli_SAG_bowtie_mapping_summary.png|1100px]] '''1000reads BLASTn + MEGAN results''' [[File:Ecoli_SAG_raw_1000reads_all_ILL_labeled.png|900px]] [[File:Ecoli_SAG_raw_1000reads_all_NX_labeled.png|900px]] [[File:Ecoli_SAG_failed2align_1000reads_all_ILL_labeled.png|900px]] [[File:Ecoli_SAG_failed2align_1000reads_all_NX_labeled.png|900px]]
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