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=== Step 2. Resuspend the SeqCap HE Universal and SeqCap HE Index Oligos === * Noi prepared this step the first time she opened the kit * We have HE Oligo Index set A # Spin the lyophilized oligo tubes, contained in the SeqCap HE-Oligo KitA briefly to allow the contents to pellet to the bottom of the tube. # Add 120ul PCR-grade H2O to SeqCap HE Universal Oligo tube (1000uM final concentration). # Add 10ul PCR-grade H2O to SeqCap HE Index Oligo tube (1000uM final concentration). # Vortex the oligos plus H2O for 5s and spin down the resuspended oligo tube. # After use, they should be stored at -15 to -25C
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