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==ligation to methylated adaptor== *Use the methylated adapter with paired-end pcr primer pairs(PCR primer PE1.0 and PCR primer PE 2.0) provided in illumina's paired end genomic DNA sample prep kit *Use 10ul of methylated adapter to 5ug gDNA insert, if starting DNA amount is less than 5ug, titrate the volume of adapter reagent accordingly to maintain 10:1 ratio of DNA {| {{table}} | align="center" style="background:#f0f0f0;"|'''Content''' | align="center" style="background:#f0f0f0;"|'''Volume''' |- | KAPA 5x ligation buffer||11.43 |- | KAPA DNA Ligase||5.71 |- | Methylation Adapter Oligo||10 |- | A-Tailed DNA||30 |- | Total||57.14 |} *Incubate at 20C for 15mins *Minelute w 20ul EB Buffer *Keep 1ul for gel image
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