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===PCR=== Check the size distribution of the PCR products with 1ul of ligation products first Template 1ul 2x iProof mix 50ul Solexa_PCR_up (10uM) 0.4ul Solexa_PCR_lo_PE (10uM) 0.4ul H2O 49ul 50X SYBG I 0.4ul 98C 30sec -> 13 cycles of (98C 10sec -> 62C 20sec -> 70C 15 sec) [[Image:ZhangLab_2 2009-10-09 15hr 40min.jpg]] Repeat the PCR with real samples Template 5ul 2x iProof mix 50ul Solexa_PCR_up (10uM) 0.4ul Solexa_PCR_lo_PE (10uM) 0.4ul H2O 45ul 50X SYBG I 0.4ul 98C 30sec -> 11 cycles of (98C 10sec -> 62C 20sec -> 70C 15 sec) gel size selection: [[Image:ZhangLab_2 2009-10-11 15hr 37min.jpg]] [[Image:ZhangLab_2 2009-10-11 15hr 38min.jpg]] [[Image:ZhangLab_2 2009-10-11 16hr 05min.jpg]]
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