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==Select Hybrid capture with SureSelect== #estimate the volume of hybridization mix that remained after 24-72hr incubation #add hybridization mix directly from the thermal cycler to the bead solution, and invert the tube to mix 3-5 times #incubate the hybrid-capture/bead solution on a nutator or shaker at RT for 30min (make sure the sample is properly mixed) #briefly spin in a centrifuge #separate the beads and buffer on a magnetic separator and remove the supernatant #Resuspend the beads in 500ul of SureSelect Wash buffer #1 by mixing on a vortex for 5 sec #incubate the samples for 15 min at RT #wash the beads: ##separate the beads and buffer on magnetic separator and remove the supernatant ##resuspend the beads in 500ul of prewarmed SureSelect Wash Buffer #2 and mix on vortex for 5 sec ##incubate the samples for 10min at 65C ##invert the tube to mix as the beads may be settled ##repeat the above steps (8.1-8.4) for a total of 3 washes, and make sure all the wash buffer has been removed #mix the beads in 50ul of SureSelect Elution buffer on a vortex mixer for 5 sec to resuspend the beads #incubate the samples for 10 min at RT #separate the beads and buffer on Dynal magnetic separator #transfer the supernatant to a new 1.5ml tube and add 50ul SureSelect Neutralization buffer to captured DNA #Desalt the capture solution with Qiagen Minelute column and elute in 15ul EB
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