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===Sequencing=== * Both fractions, 100-200 and 200-800 bp, were quantified by Qubit and qPCR. The 100-200 bp fraction had ~16 times higher concentration than the 200-800 bp, which corresponds to the gel intensity. * This indicates that the P5 and P7 regions are intact, although the smaller size fraction is too small to have actual inserts. * The 200-800 bp fraction was run on a MiSeq. The flow cell clustered at 935k/mm^2, but there were no clusters that passed filter. * The cycle intensities were extremely erratic. * If the machine was able to quantify cluster density, then the first base must have been incorporated. * It does not make sense why the sequencing would have failed.
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